Identification of Nitrogen-Fixing Genes and Gene Clusters from Metagenomic Library of Acid Mine Drainage
نویسندگان
چکیده
منابع مشابه
Identification of Nitrogen-Fixing Genes and Gene Clusters from Metagenomic Library of Acid Mine Drainage
Biological nitrogen fixation is an essential function of acid mine drainage (AMD) microbial communities. However, most acidophiles in AMD environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in AMD microbial communities. In this study, we used metagenomic sequencing to isolate nif genes in the AMD microbial...
متن کاملPreventing acid mine drainage from mine tailings
Mining activity always has high impact on its surroundings, being waste deposition the cause of multiple and complex problems to the environment. One of them is Acid Mine Drainage (AMD), causing acid water percolation through large areas in the mine site. Portugal was a prosper tungsten producer from the beginning of the XX century, having its apogee during World War II. Nowadays there are mill...
متن کاملMetagenomic and Metaproteomic Analysis of Viruses from an Acid Mine Drainage System
Project Goals (Abstracts 143-145): The aim of this project is to develop integrated genomic and proteomic (proteogenomic) methods to study virus-host dynamics in bioreactor-grown and natural microbial biofilm communities. We will utilize simultaneous metagenomic analysis of CRISPRs (the adaptive microbial immune system) and viruses to examine the co-evolution and dynamics of viruses and their m...
متن کاملRubisCO gene clusters found in a metagenome microarray from acid mine drainage.
The enzyme responsible for carbon dioxide fixation in the Calvin cycle, ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), is always detected as a phylogenetic marker to analyze the distribution and activity of autotrophic bacteria. However, such an approach provides no indication as to the significance of genomic content and organization. Horizontal transfers of RubisCO genes occurring...
متن کاملRecovery of Metals from Acid Mine Drainage
Acid Mine Drainage (AMD) poses a severe pollution problem attributed to current and past mining activities. Low pH, high concentrations of sulphates and various heavy metals makes AMD treatment a major concern because of possible deleterious effects of the effluent on the surroundings. Treatment methods to address AMD focus on neutralizing, stabilizing and removing problem pollutants through va...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0087976